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Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations |
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| Authors: | Maryam Kavousi Heidari Mehrab Pourmadadi Fatemeh Yazdian Hamid Rashedi Sayed Ali Seyyed Ebrahimi Zohreh Bagher Mona Navaei-Nigjeh Bibi Fatemeh Haghirosadat |
| Institution: | 1. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Writing - original draft (lead), Writing - review & editing (equal);2. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Methodology (equal);3. Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran;4. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran;5. Advanced Magnetic Materials Research Center, School of Metallurgy and Materials, College of Engineering, University of Tehran, Tehran, Iran;6. ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran Contribution: Formal analysis (equal), Methodology (equal), Validation (equal), Visualization (equal), Writing - review & editing (equal);7. Pharmaceutical Science Research Center, The Institute of Pharmaceutical Science (TIPS), Tehran University of Medical Science, Tehran, Iran Contribution: Data curation (equal), Methodology (equal);8. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Contribution: Methodology (equal) |
| Abstract: | Silk fibroin (SF), extracted from Bombyx mori, has unique physicochemical properties to achieve an efficient wound dressing. In this study, reduced graphene oxide (RGO)/ZnO NPs/silk fibroin nanocomposite was made, and an innovative nanofiber of SF/polyvinyl alcohol (PVA)/RGO/ZnO NPs was ready with the electrospinning technique and successfully characterized. The results of MIC and OD analyses were used to investigate the synthesized materials' antibacterial effects and displayed that the synthesized materials could inhibit growth against Staphylococcus aureus and Escherichia coli bacteria. However, both in vitro cytotoxicity (MTT) and scratch wound studies have shown that RGO/ZnO NPs and SF/PVA/RGO/ZnO NPs are not only non-toxic to NIH 3T3 fibroblasts, but also can cause cell viability, cell proliferation, and cell migration. Furthermore, improving the synthesized nanofiber's structural properties in the presence of RGO and ZnO NPs has been confirmed by performing tensile strength, contact angle, and biodegradation analyses. Also, in a cell attachment analysis, fibroblast cells had migrated and expanded well in the nanofibrous structures. Moreover, in vivo assay, SF/PVA/RGO/ZnO NPs nanofiber treated rats and has been shown significant healing activity and tissue regeneration compared with other treated groups. Therefore, this study suggests that SF/PVA/RGO/ZnO NPs nanofiber is a hopeful wound dressing for preventing bacteria growth and improving superficial wound repair. |
| Keywords: | antibacterial activity proliferation silk fibroin wound healing zinc oxide nanoparticles |