Structurally Diverse Metabolites from the Marine-Derived Streptomyces sp. DS-27 Based on Two Different Culture Conditions |
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| Authors: | Feihang Yan Jiebin Fang Wanjing Ding Xinyi Tang Xiaoming Chen Prof. Dr. Zhongjun Ma Dr. Jinhui Wang |
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| Affiliation: | 1. Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, Zhoushan, 316021 China;2. Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, Zhoushan, 316021 China Hainan Institute of Zhejiang University, Sanya, 572025 China |
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| Abstract: | Nine new compounds, including streptothiomycin A−E ( 1 – 5 ), two cyclopentenones ( 6 , 7 ), one α-pyrone ( 8 ), wailupemycin Q ( 20 ), along with sixteen known compounds were identified from a rhizosphere strain Streptomyces sp. DS-27 derived from the marine cordgrass Spartina alterniflora under two different culture conditions. All of the structures were elucidated by extensive analysis of 1D/2D NMR and HR-ESI-MS data. The absolute configurations were determined by NOESY analysis, ECD, specific rotation and GIAO NMR calculations, and DP4+ probability analysis. Bioactivity investigation showed that compounds 5 and 7 exhibited significant inhibitory effects on LPS-induced NO production in a dose-dependent manner, which indicates their anti-inflammatory potential. |
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| Keywords: | Streptomyces secondary metabolites NMR calculation DP4+ probability analysis anti-inflammatory activity |
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