A Homochiral Diphenylalanine Analog Based Mechanoresponsive Hydrogel: An Insight Towards Its Wound Healing Efficacy |
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Authors: | Dr. Priyanka Tiwari Vaibhav Shivhare Rishabh Ahuja Naureen Khan Durgesh Nandan Shukla Dr. Ankit K. Mishra Dr. Anindya Basu Dr. Anita Dutt Konar |
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Affiliation: | 1. Department of Applied Chemistry, Rajiv Gandhi Technological University, Bhopal, 462033 Madhya Pradesh, India Equally contributing authors.;2. Department of Applied Chemistry, Rajiv Gandhi Technological University, Bhopal, 462033 Madhya Pradesh, India;3. Faculty of Pharmacy, VNS Group of Institutions, Bhopal, 462044 Madhya Pradesh, India;4. School of Pharmaceutical Sciences, Rajiv Gandhi Technological University, Bhopal, India University Grants Commission, New Delhi –, 110002 New Delhi, India |
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Abstract: | Deciphering the most promising strategy for the evolution of potential wound-healing therapeutics is one of the greatest challenging affairs to date. The development of peptide-based smart scaffolds with innate antimicrobial, anti-inflammatory, and antioxidant properties is an appealing way out. Aligned to the goal a set of Hydrogelators I–IV were developed utilizing the concept of chiral orchestration in diphenylalanine fragment, such that the most potent construct with all the bench marks namely mechanoresponsiveness, biocompatibility, consistent antimicrobial and antioxidant properties, could be fished out from the design. Interestingly, our in vitro Antifungal and Lipid peroxidation analysis identified the homochiral isomer Boc-δ-Ava-L-Phe-L-Phe-OH (Hydrogelator I ), as an ideal candidate for the wound healing experiment, so we proceeded for the in vivo histopathological and antioxidant measurements in Wister rats. Indeed the wound images obtained from the different sets of animals on the 14th day of treatment demonstrated that with increased recovery time, hydrogelator I displayed a significant reduction in the lesion diameter compared to the marketed drug, and negative control. Even the histopathological measurements using H & E staining demonstrated diminished tissue destruction, neutrophil infiltration necrosis, and lymphatic proliferation in the hydrogelators, in comparison to others, backed by in vivo lipid peroxidation data. Overall our investigation certifies hydrogelator I as an effective therapeutic for managing the wound healing complication. |
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Keywords: | antioxidant property biocompatible lipid peroxidation assay mechanoresponsive hydrogelators in vivo applications |
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