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In Vitro Anthelminthic Activity and Ultrastructural Analysis of Barbatic Acid against Schistosomulae and Juvenile Worms of Schistosoma mansoni** |
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| Authors: | Dr. Hianna Arely Milca Fagundes Silva Dr. Hallysson Douglas Andrade de Araújo Prof. Dr. Mônica Camelo Pessoa de Azevedo Albuquerque Dr. Maíra de Vasconcelos Lima Dr. Mônica Cristina Barroso Martins Prof. Dr. Luiz Carlos Alves Prof. Dr. Fábio André Brayner Prof. Dr. André de Lima Aires Prof. Dr. Ana Maria Mendonça de Albuquerque Melo Prof. Dr. Nicácio Henrique da Silva |
| Affiliation: | 1. Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670-901 Recife, PE, Brazil;2. Instituto Keizo Asami (iLIKA), Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670–901 Recife, PE, Brazil Centro de Ciências da Saúde, Área Acadêmica de Medicina Tropical, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670–901 Recife, PE, Brazil;3. Departamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670-901 Recife, PE, Brazil Departamento de Energia Nuclear, Centro de Tecnologia e Geociências, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670-901 Recife, PE, Brazil;4. Departamento de Parasitologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670-901 Recife, PE, Brazil;5. Centro de Ciências da Saúde, Área Acadêmica de Medicina Tropical, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670–901 Recife, PE, Brazil Departamento de Parasitologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50670-901 Recife, PE, Brazil |
| Abstract: | Schistosomiasis affects about 260 million people worldwide and the search for new schistosomicidal compounds is urgent. In this study we evaluated the in vitro effect of barbatic acid against schistosomulae and young worms of Schistosoma mansoni. The barbatic acid was evaluated through the bioassay of motility and mortality, cellular viability and ultrastructural analysis of juvenile stages through Scanning Electron Microscopy. Barbatic acid showed a schistosomicidal effect against schistosomulae and young worms of S. mansoni after 3 h of exposure. At the end of 24 h, barbatic acid showed 100 %, 89.5 %, 52 % and 28.5 % of lethality for schistosomulae at the concentrations of 200, 100, 50 and 25 μM, respectively. For young worms, barbatic acid showed 100 % and 31.7 % of lethality at the concentrations of 200 and 100 μM, respectively. Motility changes were observed at all sublethal concentrations. There was a significant reduction in the viability of young worms after exposure to barbatic acid at 50, 100 and 200 μM. Extensive damage to the schistosomulae and young worm's tegument, was observed from 50 μM. This report provides data showing the schistosomicidal effect of barbatic acid on schistosomulae and young worms of S. mansoni, causing death, motility changes and ultrastructural damage to worms. |
| Keywords: | lichen Cladia aggregata developmental stages (schistosomulae and young worms) Schistosoma mansoni |