Phenolic Profiling,Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia |
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| Authors: | Hania Hamrouni Khadija Ben Othman Hasnia Benmoussa Sourour Idoudi Hanen Najjaa Mohamed Neffati Walid Elfalleh |
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| Institution: | 1. Energy, Water, Environment and Process Laboratory, LR18ES35), National Engineering School of Gabes, University of Gabes, Zrig Eddakhlania, 6072 Gabes, Tunisia;2. Energy, Water, Environment and Process Laboratory, LR18ES35), National Engineering School of Gabes, University of Gabes, Zrig Eddakhlania, 6072 Gabes, Tunisia
Research Unit Advanced Materials, Applied Mechanics, Innovative Processes and Environment, UR22ES04), Higher Institute of Applied Sciences and Technology of Gabes (ISSATG), University of Gabes, 6072 Gabes, Tunisia;3. Laboratory of Environmental Process Engineering, Faculty of Chemistry, BP1505, Oran-Mohamed Boudiaf University of Science and Technology, El M'naouer, 31000 Oran, Algeria;4. Laboratory of pastoral ecosystem and valorization of spontaneous plants and associated microorganisms, Institute of Arid Lands of Medenine, Route Eljorf, 4119 Medenine, University of Gabes, Tunisia |
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| Abstract: | Phytochemical screening of aqueous extract from six medicinal wild plants grown in South-eastern of Tunisia: Atriplex halimus, Teucrium polium, Moricandia arvensis, Deverra tortuoa, Haplophyllum tuberculatum and Polygonum equisetiforme were evaluated. Both decoction and ultrasound assisted extraction were used. Antioxidant, antibacterial proprieties, and phenolic profiling, using LC-ESI-MS method, were assessed. Total polyphenols, flavonoids, and condensed tannins contents ranged from 7.47±0.19 to 22.25±0.49 mg GAE/g Dw, 5.47±0.06 to 7.55±0.07 mg RE/g Dw, and 0.33±0.02 to 19.43±0.64 mg TAE/g Dw, respectively. Moreover, the reducing power and DPPH tests showed that P. equisetiforme (EC50: 12.50±0.50 μg/ml; DPPH?+: 213.49±4.24 mg TEAC/g DW), T. polium (EC50: 25.00±1.00 μg/ml; DPPH?+: 181.39±9.47 mg TEAC/g DW) as well as H. tuberculatum (EC50: 56.25±0.25 μg/ml; DPPH?+: 177.83±5.85 mg TEAC/g DW) extracts were the most effective natural antioxidants. For anti-bacterial activity, the ultrasonic extract of H. tuberculatum showed the highest activity against both P. aeruginosa (13.50±0.71 mm) and S. aureus (13.00±0.00 mm) at 10 mg/ml. Furthermore 24 phenolic compounds were identified, with predominance of quinic acid, gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, trans-ferulic acid, catechin (+), trans-cinnamic and silymarin. These results were further consolidated by to heatmap clustering with P. equisetiforme, H. tuberculatum, T. polium as the main antioxidant and antibacterial sources which supports their domestication and industrial use. |
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| Keywords: | medicinal plants phenolic compounds extraction antioxidant activity antibacterial activity |
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