Reactivation of Horseradish peroxidase in organic solvent using extraction |
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Authors: | Jin Ryoun Kim Yoo Seong Choi Young Je Yoo |
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Affiliation: | (1) School of Chemical Engineering, Seoul National University, Seoul, 151-742, Korea |
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Abstract: | A novel extraction method was developed aiming at increasing the stability of enzymes in organic solvent media. Horseradish peroxidase (HRP), inactivated in a tetrahydrofuran (THF)/water (1:1, v/v), regained and maintained its activity when HRP was extracted by adding a THF/benzene mixture to the original solution. However, the HRP activity was drastically lowered in the enzyme-free blank solution that had been formed by employing the same extraction procedure. As a result, the reactivation after the extraction is believed to depend on enzyme history, and might be arisen from an irreversible structural change of the enzyme. |
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Keywords: | enzyme extraction Horseradish peroxidase organic solvent reactivation |
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