Spin labelling of Bacillus anthracis endospores: a model for in vivo tracking by EPR imaging |
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| Authors: | Tsai Pei Cao Guan-Liang Merkel Tod J Rosen Gerald M |
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| Institution: |
a Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, USA
b Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD, USA
c Center for EPR Imaging In Vivo Physiology, University of Maryland School of Pharmacy, Baltimore, MD, USA
d Laboratory of Respiratory and Special Pathogens, Center for Biologics Evaluation and Research, MD, USA |
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| Abstract: | Anthrax is caused by the gram-negative bacterium, Bacillus anthracis. Infection by this microbe results from delivery of the endospore form of the bacillus through direct contact, either topical or inhalation. With regard to the latter route of administration, it is proposed that endospores of B. anthracis enter the lungs and are phagocytized by host alveolar macrophages. Thereafter, it is unclear as to how endospores travel to distal loci and what tissues are the targets. Herein, this study describes the spin labelling of endospores through two different approaches with various aminoxyls. Indeed, after exposure to RAW 264.7 cells, these aminoxyl-containing endospores were phagocytized, as demonstrated by EPR spectroscopy of the infected macrophage, thus providing a potential tool for EPR imaging in animals. |
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| Keywords: | Bacillus anthracis electron paramagnetic resonance spectroscopy maleimide-containing aminoxyls acetoxymethoxycarbonyl-containing aminoxyls |
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