Extracellular deoxyribonuclease production by a thermophile, Streptomyces thermonitrificans |
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Authors: | S S Deshmukh V Shankar |
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Institution: | (1) Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India, IN |
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Abstract: | A thermophilic bacterial strain, Streptomyces thermonitrificans, produced high levels of extracellular deoxyribonuclease (DNase) when grown on NBG medium (containing 1% peptone, 0.3% beef
extract, 1% glucose and 0.5% NaCl). Maximum DNase activity (140 U ml−1) was obtained, in 24 h, when the culture was grown on modified NBG medium (containing 1.3% beef extract, 1% glucose, 0.5%
NaCl and 50 μM Mn2+ at 45°C. The crude enzyme showed higher activity on native DNA than on sonicated and heat denatured DNA. Moreover, addition
of Mn2+ in the assay mixture resulted in a significant stimulation (10–15 fold) of the enzyme activity.
Received 24 November 1998/ Accepted in revised form 25 April 1999 |
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Keywords: | : Streptomyces thermonitrificans thermophilic Streptomyces sp extracellular DNase enzyme production metal ions |
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