The major glutathione S-transferase in salmonid fish livers is homologous to the mammalian pi-class GST. |
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Authors: | R J Dominey I A Nimmo A D Cronshaw J D Hayes |
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Institution: | University Department of Clinical Chemistry, Royal Infirmary, Edinburgh, Scotland, UK. |
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Abstract: | 1. The hepatic glutathione S-transferase (GST) isoenzymes were isolated and characterized from salmon, sea trout and rainbow trout. 2. In all three species the predominant GST expressed comprised subunits of Mr 24,800. These subunits each co-migrated with the rat pi-class Yf polypeptide during SDS/polyacrylamide gel electrophoresis. 3. Western blotting experiments demonstrated immunochemical cross-reactivity between the major salmonid and the rat pi-class GSTs. 4. The salmon GST of subunit Mr 24,800 was digested with cyanogen bromide and the peptides, once purified by reverse-phase HPLC, were subjected to automated amino acid sequencing. 5. Over the region sequenced, the salmon GST possessed about 65% homology with the rat and human pi-class GST. |
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