Functional analysis of the Rubisco large subunit ÂN-methyltransferase promoter from tobacco and its regulation by light in soybean hairy roots |
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Authors: | M Mazarei Z Ying R L Houtz |
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Institution: | (1) Department of Horticulture and Landscape Architecture, Plant Physiology/Biochemistry/Molecular Biology Program, University of Kentucky, Lexington, KY 40546, USA Fax: +1-606-2572859 e-mail: rhoutz@ca.uky.edu, US |
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Abstract: | The ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco) large subunit (LS)
ɛ
N-methyltransferase (Rubisco LSMT) catalyzes post-translational methylation of the ɛ-amino group of lysine-14 in the LS of Rubisco. The entire nucleotide sequence for the tobacco (Nicotiana tabacum) Rubisco LSMT (rbcMT-T) gene including the putative promoter region was recently reported, and sequence analysis of the promoter
region revealed seven GT-1 motifs. The ability of several truncated rbcMT-T promoter fragments to confer light responsiveness
to reporter gene expression in transgenic soybean (Glycine max) hairy roots was examined. Chimeric constructs consisting of the rbcMT-T promoter fused to a bacterial β-glucuronidase (GUS) reporter gene and transferred to soybean via Agrobacterium rhizogenes were evaluated. The rbcMT-T promoter fragments conferred expression of the reporter gene in transgenic hairy roots, callus,
and cell suspension cultures based on histochemical and fluorometric GUS analyses. The results suggest a quantitative role
for the number of GT-1 motifs in determining differential expression between light and dark conditions.
Received: 7 January 1998 / Revision received: 23 March 1998 / Accepted: 13 April 1998 |
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Keywords: | Agrobacterium rhizogenes Rubisco ɛ N-methyltransferase Promoter GT-1 |
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