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Editing of Multidimensional NMR Spectra of Partially Deuterated Proteins. Measurement of Amide Deuterium Isotope Effects on the Chemical Shifts of Protein Backbone Nuclei |
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| Authors: | Axel Meissner Jacques Briand Ole Winneche Sφrensen |
| Institution: | (1) Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark;(2) Department of Physical and Structural Chemistry, UW-2940, SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, P.O. Box 1539, King of Prussia, PA, 19406-0939, U.S.A |
| Abstract: | Novel multidimensional NMR pulse sequences are presented for determination of amide deuterium isotope effects on the 13C chemical shifts of the backbone in proteins. The sequences edit heteronuclear triple resonance spectra into two subspectra according to whether a deuterium or a proton is attached to 15N for the pertinent correlations. The new experiments are demonstrated using 13C, 15N-labeled RAP 17-112 (N-terminal domain of  2-macroglobulin receptor associated protein). |
| Keywords: | editing isotope effects multidimensional NMR ISP |
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