Effect of elevated temperature on catalase and superoxide dismutase during maize development

Seeds of the inbred maize lines, W64A, R6-67, and D10, were germinated and grown at 25 degrees, 35 degrees, or 40 degrees C for up to 10 days. The catalase activity in scutella of W64A seedlings grown at 40 degrees C was slightly lower than that in seedlings grown at 25 degrees C. The total superoxide dismutase activity in scutella was lower in seedlings grown at 40 degrees C than in those grown at 25 degrees C during the first 3 days of germination, but thereafter was not significantly different at these temperatures. The high-catalase mutant lines, R6-67 and D10, grown at 40 degrees C exhibited a developmental pattern of catalase activity that was severalfold lower than that seen in seedlings grown at 25 degrees C. The decrease in catalase activity in R6-67 seedlings grown at 40 degrees C was correlated with lower amounts of CAT-2 protein, which is normally present at significantly high levels in this line. The application of a catalase synthesis inhibitor revealed that the low levels of CAT-2 in R6-67 grown at 40 degrees C were due to slightly higher degradation rates and a significant drop in the rate of catalase protein synthesis.