杜鹃红山茶遗传多样性的ISSR分析

运用ISSR分子标记技术,利用筛选的10条ISSR引物,对珍稀濒危植物杜鹃红山茶(Camellia changii Ye)2个亚种群60个单株的遗传多样性进行了研究。结果表明:物种水平上的多态位点百分率(PPB)为55.29%,Nei’s基因多样性指数(H)及Shannon多态性信息指数(I)分别为0.2191和0.3215。种群间的遗传分化系数(GST)仅为0.0922。研究结果揭示了杜鹃红山茶的遗传多样性较低,亚种群间遗传分化较小。小种群和人为活动干扰是杜鹃红山茶现存种群的主要限制因素。影响杜鹃红山茶种群发展的其它因素亟需进一步研究。

Genetic Diversity of Camellia changii Ye (Theaceae) Using ISSR Markers

Camellia changii Ye is a rare and endangered species endemic to China. It is confined to the E'huangzhang Nature Reserve at Yangchun City, southwestern Guangdong Province, South China. The genetic diversity was studied using Inter-Simple Sequence Repeat (ISSR) markers. A total of 60 individuals from two subpopulations were sampled. Ten primers with discernible DNA bands were applied. The percentage of polymorphic bands (PPB), Nei's Index and Shannon's Index were 55.29%, 0.2191 and 0.3215, respectively. The coefficient of gene differentiation (GST) was 0.0922. These results indicate that C. changii has low genetic diversity and low differentiation between the two subpopulations. Small population size and human disturbances are the main limiting factors for the extant population. Further studies on the factors limiting population development are urgently required.