毛竹"韧皮部结"发育过程中Ca2+-ATP酶的超微定位

用电镜和细胞化学技术对毛竹[Phyllostachys edulis（Carr.）H.De Lehaie]节部“韧皮部结”发育过程中Ca^2＋-ATP酶进行了超微细胞化学定位研究.结果显示：在“韧皮部结”形成期,仅细胞质膜和细胞核上具有很高的Ca^2＋-ATP酶活性;随着“韧皮部结”的发育,发育期细胞质膜上的Ca^2＋-ATP酶活性较形成期有所降低,而细胞核上仍保持较高的Ca^2＋-ATP酶活性,胞间连丝、运输小泡膜上都具有Ca^2＋-ATP酶活性;发育后期,液泡膜及内质网上也开始出现Ca^2＋-ATP酶沉积物;成熟期的“韧皮部结”细胞质膜上的Ca^2＋-ATP酶活性较发育期有所升高,并且在“韧皮部结”成熟的过程中,细胞核、内质网、胞间连丝、质体膜和细胞质降解物上始终都有较高的Ca^2＋-ATP酶活性.实验结果表明“韧皮部结”细胞具有活跃的生理代谢以及频繁的共质体运输和信息交流.

Ultracytochemical Localization of Ca2+-ATPase during the Phloem Ganglion Development in Phyllostachys edulis

The Ultracytochemical localization of Ca2+-ATPase during the phloem ganglion development in Phyllostachys edulis(Carr.) H.De Lehaie was studied with cytochemical technology.In the initiating phase of the phloem ganglion,the plasma membrane and nuclei have high Ca2+-ATPase activity.During the developmental phase,Ca2+-ATPase deposits in the plasma membrane decreased,but Ca2+-ATPase activity in the nuclei remained high.Ca2+-ATPase deposits were visualized in transfer vesicles and plasmodesmata.In the later period of the developmental phase,the distribution of Ca2+-ATPase appeared in the tonoplast and ER(endoplasmic reticulum).During the maturation phase,Ca2+-ATPase activity in the plasma membrane is little higher than that of in developmental phase,and during the maturation of the phloem ganglion,the nuclei,plasmodesmata,ER,plastid membrane and degradation always have high Ca2+-ATPase activity.These findings indicate that the phloem ganglion has active physiological metabolism,symplastic translocation and communication.