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A specific rearrangement of mitochondrial DNA induced by tissue culture |
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| Authors: | T Brears G J Curtis D M Lonsdale |
| Institution: | (1) Institute of Plant Science Research (Cambridge Laboratory), Maris Lane, CB2 2JB Trumpington, Cambridge, UK;(2) Present address: Plant Breeding International, Maris Lane, CB2 2LQ Trumpington, Cambridge, UK;(3) Present address: Semper Biotechnology, 106 London Street, RG1 4SJ Reading, UK |
| Abstract: | Summary The induction, growth and regeneration of sugar beet callus to whole plants were all found to be highly genotype-specific. Regenerants of one line (of sterile cytoplasm) were obtained and a study of the chloroplast and mitochondrial DNA in these somaclones was undertaken by gel electrophoresis and cosmid hybridization. In one somaclone a rearrangement in the mitochondrial genome was observed; the novel arrangement of this part of the genome was identical to the corresponding area of the genome of the normal cytoplasm though it was otherwise of sterile type. This suggests that mitochondrial DNA may have a propensity to undergo certain types of rearrangement. |
| Keywords: | Mitochondrial DNA Somaclonal variation |
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