Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid |
| |
Authors: | Li Ang Chen LiangLiang Ren HaiYun Wang XueChen Zhang HaiWen and Huang Rong-Feng |
| |
Institution: | (1) Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China;(2) National Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100094, China;(3) College of Life Sciences, Beijing Normal University, Beijing, 100875, China;(4) National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, 100081, China |
| |
Abstract: | In rice, the characterization of OsEBP-89 is inducible by various stress-or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought
and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple
potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression
under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible
by MeJA in tobacco leaves. To further determine the crucial sequences responsible for MeJA induction, we generated a series
of deletion promoters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene
driven by these mutant promoters show that the essential region for MeJA induction is positioned in the region between −1200
and −800 in OsEBP-89 promoter containing a G-box (−1127), which is distinct from the essential region containing ERE (−562) for ACC induction.
In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli. OsEBP-89, essential DNA region, methyl jasmonic acid, transient assay, promoter, tobacco leaves
Contributed equally to this work
Supported by the National Basic Research Program of China (Grant No. 2006CB101700) and the National Natural Science Foundation
of China (Grant Nos. 30671135, 30525034 and 30730060) |
| |
Keywords: | OsEBP-89 essential DNA region methyl jasmonic acid transient assay promoter tobacco leaves |
本文献已被 SpringerLink 等数据库收录! |
|