| Vc二步发酵伴生菌巨大芽孢杆菌的选育 |
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| 引用本文： | 张舟,江晶.Vc二步发酵伴生菌巨大芽孢杆菌的选育[J].微生物学杂志,1999,19(2):8-10. |
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| 作者姓名： | 张舟 江晶 |
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| 作者单位： | 中科院沈阳应用生态研究所!110015(张舟，冯树，王勇，张忠泽)，东北制药总厂(江晶) |
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| 基金项目： | 省、市重点攻关项目，中国科学院“百人计划”资助 |
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| 摘 要： | 巨大芽孢杆菌经紫外线诱变，获得2株耐低pH、抗KGA的菌株：Bn，B5。在pH6.7～7．0的发酵培养基中与氧化葡萄糖酸杆菌发酵，Bn和B5的平均糖酸转化率分别提高3.5％，3．3％.在pH6．2的发酵培养基中，平均糖酸转化率提高11．4％，12．3％。在pH6．2和pH7．0含3％KGA的培养基中，2菌提前3～6h到达对数生长期，稳定期延长3～6h.经连续30代转接，特性稳定。
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| 关 键 词： | Vc二步发酵 选育 巨大芽孢杆菌 |
BREEDING OF ASSOCIATED BACTERLA Bacillus megaterium IN "TWO-STEP FERMENTATION" OF VITAMIN C |
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| Zhang Zhou et al..BREEDING OF ASSOCIATED BACTERLA Bacillus megaterium IN "TWO-STEP FERMENTATION" OF VITAMIN C[J].Journal of Microbiology,1999,19(2):8-10. |
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| Authors: | Zhang Zhou |
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| Abstract: | Two mutant strains (Bn, B5) with low pH and KGA tolerance of fucillus megateruim wereobtained by mutation with ultraviolet light. Their converting rate of L-sorbose to KGA was increased by 3. 5 % and 3. 3% respcctivcly in medium of pH 6. 7-7.0 and by 11. 4 % and 12. 3%in medurn of pH 6. 2 when they were ctiltured with Gluconobacter oxydans. Bn and B5 went mtolog phase 3-6 hours in advance and grew in stationary phase longer 3-6 hours than CK in medium of pH 6. 2 and pe 7. 0 with 3 % of KGA. The charactcristics of Bn and B5 were shown stableafter oontinuously transferred for 30 generations. |
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| Keywords: | two-step fermentation of Vc breeding Bacillus megaterium |
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