Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: Phylogenetic implications |
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Authors: | Anna Maria Sanangelantoni Daniela Barbarini Giuseppe Pasquale Piero Cammarano Orsola Tiboni |
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Affiliation: | (1) Dipartimento di Genetica e Microbiologia A. Buzzati Traverso, Via S. Epifanio 14, I-27100 Pavia, Italy;(2) Dipartimento di Biopatologia Umana University La Sapienza, Viale Regina Elena 324, I-00161 Roma, Italy |
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Abstract: | Summary The glnA gene of the thermophilic sulphur-dependent archaebacterium Sulfolobus solfataricus was identified by hybridization with the corresponding gene of the cyanobacterium Spirulina platensis and cloned in Escherichia coli. The nucleotide sequence of the 1696 bp DNA fragment containing the structural gene for glutamine synthetase was determined, and the derived amino acid sequence (471 residues) was compared to the sequences of glutamine synthetases from eubacteria and eukaryotes. The homology between the archaebacterial and the eubacterial enzymes is higher (42%–49%) than that found with the eukaryotic counterpart (less than 20%). This was true also when the five most conserved regions, which it is possible to identify in both eubacterial and eukaryotic glutamine synthetases, were analysed. |
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Keywords: | Glutamine synthetase Nuxleotide sequence Archaebacteria Phylogeny |
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