大气污染对大鼠呼吸道微生态的影响研究

探讨了大气污染引起呼吸道症状和机体健康效应的作用机理,为防治大气污染及其对机体的损害提供理论依据。采用模拟现实大气污染的方法,分高、中、低浓度用Wistar大鼠制备大气污染动物模型,对大鼠口咽部菌群进行定性、定量、定位检测,动态研究。大气污染大鼠模型口咽部微生态发生改变。与污染前比,肺炎克雷伯杆菌等致病菌检出率明显增加(P<0.05),厌氧菌含量明显减少(P<0.05),并且随着染毒时间厌氧菌和细菌总量都呈明显下降趋势(P<0.05);口咽部菌群含量在一定范围内波动,没有随着染毒浓度改变而呈明显改变。大气污染对呼吸道正常菌群有毒害作用,对微环境有破坏作用,导致微环境改变,正常菌群含量减少,致病菌定植,是呼吸道疾病发病率升高的重要因素之一。

Effect of Air Pollution on Microecology of Rat Respiratory Tract

In order to explore the mechanism of respiratory tract symptom and health effect caused by air pollution and provide theoretical basis for preventing air pollution,animal model of Wistar rat was made and rats were poisoned by imitating real air pollution,including high,middle,and lower air pollution concentration.Bacterial flora of pharynx of was qualitatively,quantitatively and position measured and dynamic analyzed.The results showed that microecology of pharynx of rats in polluted environment appeared change.Klebsiella pneumoniae and other bacteria increased and anaerobic bacteria decreased(P<0.05).The number of anaerobic bacteria and whole bacteria declined with polluting time extending. The density of pharynx bacterial flora fluctuated in a certain range and did not change with pollutant concentration.Therefore,air pollution could lead to the change of bacterial flora in respiratory tract and damage the microenvironment,make normal bacteria of pharynx decreased and pathogenic bacteria planted,and it is an important factor to increase incidence of respiratory disease.