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An efficient method for preparing high quality DNA from plant DNA libraries in lambda phage |
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| Authors: | Domenica R. Massardo Grazia M. Borrelli Natale Di Fonzo Pietro Alifano Luigi Del Giudice |
| Affiliation: | (1) Istituto di Genetica e Biofisica Adriano Buzzati Traverso, CNR, via G. Marconi 10, 80125 Napoli, Italy;(2) Istituto Sperimentale per la Cerealicoltura, Sezione di Foggia, S.S, 16 Km 675, 71100 Foggia, Italy;(3) Dipartimento di Biologia, Università degli Studi di Lecce, via Monteroni, 73100 Lecce, Italy |
| Abstract: | An efficient method has been developed to improve preparation of phage particles by ammonium sulfate precipitation and to yield high quality DNA. The method, that has been used to screen plant DNA libraries constructed in  vectors, is inexpensive, does not require purification of phage particles, and can be used from either plate stocks or liquid lysates. Up to 1100  g DNA was produced from 5 ml lysate obtained from agar plates. |
| Keywords: | ammonium sulfate cDNA library |
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