Photoautotrophic growth response of in vitro cultured coffee plantlets to ventilation methods and photosynthetic photon fluxes under carbon dioxide enriched condition |
| |
Authors: | Thi Nguyen Quynh Kozai Toyoki Heo Jeongwook Xuan Thai Du |
| |
Institution: | (1) Laboratory of Environmental Control Engineering, Faculty of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan;(2) Laboratory of Plant Cell Technology, Institute of Tropical Biology, NCST-VN, 1 Mac Dinh Chi Street, Ho Chi Minh City, Vietnam;(3) Research Center for the Development of Advanced Hortechnology, Chungbuk National University, Chungbuk, Cheongju, 361-763, Republic of Korea;(4) Laboratory of Plant Cell Technology, Institute of Tropical Biology, NCST-VN, 1 Mac Dinh Chi Street, Ho Chi Minh City, Vietnam |
| |
Abstract: | Effects of two ventilation methods (forced and natural) and two photosynthetic photon fluxes (PPF, 150 and 250 μmol m−2 s−1) on the photoautotrophic growth of in vitro cultured coffee (Coffea arabusta) plantlets were investigated. Number of air exchanges was 2.7, 5.9 and 3.9 h−1 for forced low rate, forced high rate and natural ventilation, respectively. Single node cuttings of in vitro cultured coffee plantlets were cultured on Florialite, a mixture of vermiculite and cellulose fibers with high air porosity,
emerged in liquid half strength basal MS medium, without sucrose, vitamins and plant growth regulators. The study included
40 days in the in vitro stage and 10 days in the ex vitro stage. Mean fresh and dry weights, leaf area, shoot and root lengths and net photosynthetic rate per plantlet were significantly
greater in forced high rate treatments compared with those in natural and forced low rate treatments. PPF had a distinct effect
on shoot length suppression and root elongation of coffee plantlets in forced high rate treatments. The control of carbon
dioxide concentration inside the culture box according to the plant demand when growing was easy with the forced ventilation
method in photoautotrophic micropropagation.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | CO2 enriched condition flow rate forced ventilation natural ventilation number of air exchanges photoautotrophic micropropagation |
本文献已被 SpringerLink 等数据库收录! |
|