| 核酸探针中放射性同位素的快速检测 |
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| 引用本文： | 罗文永,胡骏,刘文华,陈建伟,毛兴学,李晓方.核酸探针中放射性同位素的快速检测[J].细胞生物学杂志,2004,26(3):324-326. |
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| 作者姓名： | 罗文永 胡骏 刘文华 陈建伟 毛兴学 李晓方 |
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| 作者单位： | [1]广东省农业科学院水稻研究所农业部水稻遗传改良重点实验室及广东省水稻育种新技术重点实验室，广州510640 [2]中山大学植物基因工程教育部重点实验室，广州510275 |
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| 基金项目： | 农业部“948”项目(20020201)，广东省自然科学基金(036745) |
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| 摘 要： | 利用尼龙膜作为介质，以0．4mol／L的NaOH为层析液，进行膜上层析，分离经放射性同位素标记的核酸探针和未掺入的放射性游离单核苷酸，再经放射性强度测定即可计算出标记后的核酸探针的放射性比活。方法简单快捷，产生的放射性废物少，完全可以替代经典的三氯乙酸沉淀法及滤膜吸附法。
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| 关 键 词： | 核酸探针 放射性同位素 快速检测 核酸杂交 尼龙膜 |
| 文章编号： | 0253-9977(2004)03-324-03 |
| 修稿时间： | 2003年10月13 |
A Simple Method for Detection of Radioactivity of Nucleic Acid Probes |
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| LUO Wen Yong,HU Jun,LIU Wen Hua,CHEN Jian Wei,MAO Xin Xue,LI Xiao Fang The Key Laboratory of Rice Genetic Improvement of Ministry of Agriculture and Guangdong Key Laboratory of New Technology in Rice Breeding,Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou ,China The Key Laboratory of Gene Engineering of Ministry of Education,Zhongshan University Guangzhou ,China.A Simple Method for Detection of Radioactivity of Nucleic Acid Probes[J].Chinese Journal of Cell Biology,2004,26(3):324-326. |
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| Authors: | LUO Wen Yong HU Jun LIU Wen Hua CHEN Jian Wei MAO Xin Xue LI Xiao Fang The Key Laboratory of Rice Genetic Improvement of Ministry of Agriculture and Guangdong Key Laboratory of New Technology in Rice Breeding Rice Research Institute Guangdong Academy of Agricultural Sciences Guangzhou China The Key Laboratory of Gene Engineering of Ministry of Education Zhongshan University Guangzhou China |
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| Institution: | LUO Wen Yong,HU Jun,LIU Wen Hua,CHEN Jian Wei,MAO Xin Xue,LI Xiao Fang The Key Laboratory of Rice Genetic Improvement of Ministry of Agriculture and Guangdong Key Laboratory of New Technology in Rice Breeding,Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China The Key Laboratory of Gene Engineering of Ministry of Education,Zhongshan University Guangzhou 510275,China |
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| Abstract: | We reported here a new method for detection of radioactivity of radiolabeled nucleic acid probes.
In this method, nylon filter and 0.4 mol/L of NaOH were used as chromatgraphy media and chromatography solvent
respectively. After chromatography, radiolabeled nucleic acid probes stayed at origin, while free nucleotides mi-
grated to solvent front. Thus the radioactivity of radiolabeled nucleic acid and nucleotide could be measured
respectively. This method is very simple, and could be finished in 30 min. On the other hand, this method leads to
less contamination for the only radioactive waste was nylon filter itself after detection. |
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| Keywords: | radioactivity nucleic acid probes nylon filter |
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