The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses |
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Authors: | O-W Merten H Kallel J-C Manuguerra M Tardy-Panit R Crainic F Delpeyroux S Van der Werf P Perrin |
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Institution: | (1) Laboratoire de Technologie Cellulaire, Institut Pasteur, 25, rue du Docteur Roux, F-75724 Paris Cedex 15, France;(2) Present address: A.F.M. Généthon II, 1, rue de l'Internationale, BP 60, F-91002 Evry Cedex 2, France;(3) Service de Production des Vaccins Virologiques, Institut Pasteur de Tunis, 13, Place Pasteur, B.P. 74, 1002 Tunis Belvédère, Tunisie;(4) Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25, rue du Docteur Roux, F-75724 Paris Cedex 15, France;(5) Laboratoire d'Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, 25, rue du Docteur Roux, F-75724 Paris Cedex 15, France;(6) Laboratoire des Lyssavirus, Institut Pasteur, 25, rue du Docteur Roux, F-75724 Paris Cedex 15, France |
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Abstract: | The development of media free of serum and animal or human proteins is of utmost importance for increasing the safety of biologicals
produced for therapy and vaccination. In order to reduce the risk of contamination, we have modified the serum free medium
MDSS2, a very efficient serum free medium for the production of various biologicals including experimental vaccines using
different cell lines (Merten et al., 1994), by replacing the animal derived products by plant extracts. The new serum and
animal protein free medium (MDSS2N) can be efficiently used for biomass production of various cell lines. These cells grow
equally well or better in this new serum-free medium than in the old formulation (MDSS2):
• BHK-21/BRS cells, adapted to MDSS2N, showed an overall specific growth rate of 0.0197 h-1 (μ_max = 0.0510±0.0058 h-1), whereas
those cultivated in MDSS2 grew with an average specific growth rate of 0.0179 h-1 (μ_max = 0.0305±0.0177 h-1).
• Vero cells grew with an average specific growth rate of 0.0159 h-1 and 0.0153 h-1 in MDSS2 and MDSS2N, respectively. Very
similar growth rates were obtained in microcarrier cultures in stirred tank reactors: the specific growth rates were 0.0161
h-1 and 0.0166 h-1 for MDSS2 and MDSS2N cultures, respectively.
• For MDCK cells, when cultured on microcarriers in bioreactors, a higher average specific growth rate was observed in MDSS2N
than in MDSS2; values of 0.0248 h-1 and 0.0168 h-1, respectively, were obtained.
The capacity of MDSS2N to support the production of different viruses was equally evaluated and it could be established that
for certain viruses there are no or insignificant differences between MDSS2N and MDSS2 (influenza and polio virus), whereas,
the production of rabies virus is somewhat reduced in MDSS2N when compared to MDSS2. The use of MDSS2N for cell culture and
the production of various viruses is discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | BHK-21/BRS MDCK non-animal-derived serum-free medium Vero virus-production |
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