Laser microirradiation of cerebellar neurons in culture |
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Authors: | James E. Olson Walter Schimmerling G. Craig Gundy Cornelius A. Tobias |
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Affiliation: | (1) Department of Biophysics, University of California, 94720 Berkeley, CA;(2) Biology and Medicine Division, Lawrence Berkeley Laboratory, 94720 Berkeley, CA;(3) Department of Biophysics, University of California, 94720 Berkeley, CA;(4) Present address: Department of Neurology, School of Medicine, Stanford University, 94305 Stanford, CA;(5) Present address: Department of Zoology, Weber State College, 84408 Ogden, UT |
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Abstract: | Electrophysiological and ultrastructural effects of focused laser radiation on neurons from neonatal rat cerebellum in tissue culture are reported. Action potentials were elicited by an extracellular current pulse train. The stimulator voltage required for half-maximum response frequency was measured as a function of the energy delivered by a single laser pulse. Above a “threshold” laser energy, the cell response to stimulation became negligible for all stimulator voltages. Electron micrographs of cells revealed that the mitochondria are preferentially damaged at an energy comparable to the electrophysiological threshold. The damaged mitochondria showed swollen matrix space and disrupted cristae membranes. Higher laser energies resulted in damage to other cytoplasmic structures. The results are consistent with a model that assumes that light interaction with the nerve cells proceeds by local heating of the mitochondria and nearby structures and leads to an increased conductance of the membrane to some ionic species. |
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Keywords: | Photobiology, of cerebellar neurons in culture lasers, in irradiation of neurons mitochondria, of laser-irradiated neurons cerebellum, laser-irradiated neurons of electrophysiological effects, in laser-irradiated neurons ultrastructural effects, in laser-irradiated neurons irradiation, of neurons by lasers neurons, laser irradiation of cerebellar |
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