Effects of leptin to cultured growth plate chondrocytes |
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Authors: | Nakajima Ryoichi Inada Hiroshi Koike Tatsuya Yamano Tsunekazu |
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Affiliation: | Department of Pediatrics, Osaka City University Graduate School of Medicine, Osaka, Japan. ryoichi@msic.med.osaka-cu.ac.jp |
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Abstract: | OBJECTIVE: This study aimed to evaluate whether leptin has any effect on growth plate chondrocytes. METHODS: We studied the effects of exogenous leptin on cultured rabbit growth plate chondrocytes. This involved assessing [3H]thymidine incorporation, alkaline phosphatase (ALP) activity, proteoglycan production, leptin receptor (Ob-R) activity, and detection of Ob-R using Western blot analysis. RESULTS: The existence of Ob-R in growth plate chondrocytes was revealed by Western blot and Ob-R activity. Prior to semiconfluence, leptin increased [3H]thymidine incorporation while at the semiconfluent and early confluent stages, leptin promoted ALP activity and tended to promote proteoglycan production. CONCLUSION: Growth plate chondrocytes possess Ob-Rs, and leptin enhance chondrocyte proliferation and subsequent cell differentiation. |
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