Development and characterization of 13 novel microsatellite loci from the flat-headed bat (Tylonycteris pachypus) with cross-species amplification in closely related taxa |
| |
Authors: | Jiangxia Yin Wenchao Liu Tingting Guo Shuyi Zhang Panyu Hua |
| |
Institution: | (1) Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China;(2) School of Life Science, East China Normal University, Shanghai, 200062, China |
| |
Abstract: | 13 novel microsatellite loci was isolated using the enriched library method from genomic DNA of the flat-headed bat (Tylonycteris pachypus). These loci showed high levels of genetic polymorphism testing on 54 individuals sampled from Guangxi province, China. The
number of observed alleles per locus ranged from 2 to 15. The expected and observed heterozygosity values ranged from 0.170
to 0.900 and from 0.185 to 0.944, respectively. One loci departed significantly from Hardy-Weinberg equilibrium (HWE) after
Bonferroni correction and no Linkage disequilibrium was found between any pairs of loci. In addition, these loci were tested
in the sister species, Tylonycteris robustula, seven loci amplified successfully and were also polymorphic. |
| |
Keywords: | Tylonycteris pachypus Microsatellites Conservation |
本文献已被 SpringerLink 等数据库收录! |
|