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Quantitative determination of wood-derived soluble oligosaccharides by HPLC
Authors:J. Buchert  M. Siika-aho  M. Bailey  J. Puls  A. Valkeajärvi  J. Pere  L. Viikari
Affiliation:(1) VTT Biotechnical Laboratory, P.O. Box 202, FIN-02151 Espoo, Finland;(2) Federal Research Centre for Forestry and Forest Products, Institute of Wood Chemistry and Chemical Technology of Wood, Leuschnerstr. 91, 80 Hamburg, Federal Rebublic of Germany
Abstract:Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.
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