Enzymatic activation of the Bacillus sphaericus mosquito larvicidal toxin |
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| Authors: | E W Davidson A L Bieber M Meyer C Shellabarger |
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| Institution: | 1. Microbial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, India;2. Department of Molecular Biology and Biotechnology, Cotton University, Guwahati, Assam, India;1. iES Landau, Institute for Environmental Sciences, University of Koblenz-Landau, D-76829 Landau, Germany;2. Université Grenoble Alpes, CNRS, Laboratoire d''Ecologie Alpine, F-38000 Grenoble, France;3. Centre of Island Research and Environmental Observatory, PSL Université Paris: EPHE-UPVD-CNRS, USR 3278 CRIOBE, Université de Perpignan, F-66860 Perpignan, France;4. Tour du Valat, Research Institute for the Conservation of Mediterranean Wetlands, Le Sambuc, F-13200 Arles, France;5. Université Grenoble Alpes, CNRS, CEA, IBS, F-38000 Grenoble, France;2. Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom;1. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra 400085, India |
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| Abstract: | Bacillus sphaericus insecticidal toxin was activated by trypsin, α-chymotrypsin, and mosquito gut homogenates to a form which was cytotoxic to cultured mosquito cells. Gut extracts from highly resistant Aedes aegypti larvae were as effective in activating this toxin as extracts from the highly sensitive species Culex quinquefasciatus. Activation altered the apparent molecular weight of the toxin by ca. 2–4 kDa. |
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