Partial purification and reconstitution of the aspartate transport system from Halobacterium halobium |
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Authors: | R V Greene R E MacDonald |
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Institution: | Division of Biological Sciences, Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853 USA |
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Abstract: | Membrane vesicles of Halobacterium halobium R1Wrm bind to an aspartic acid-agarose affinity column. After disruption of the bound vesicles by low ionic strength, a protein fraction is eluted from the column with 2.5% cholate in 3 M NaCl. When this fraction is reconstituted with soybean lipids to form proteoliposomes, the proteoliposomes exhibit active aspartate accumulation. Aspartate transport in the reconstituted system is driven by a chemical sodium gradient (out greater than in), exhibits sensitivity to an electrical potential, and is specific for L-aspartate. These characteristics are consistent with observations on aspartate transport in intact membrane vesicles of H. halobium. Initial aspartate transport rates in the reconstituted system are about ninefold enhanced over the native system. The system developed should be useful in future purification schemes and studies of the molecular details of membrane transport. |
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Keywords: | To whom correspondence should be addressed |
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