Carbohydrate exposure of human promyelocytic HL60 cells and histiocytic U937 cells during phagocytic differentiation assessed with fluoresceinated lectins |
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Authors: | Karl-Erik Magnusson Olle Stendahl |
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Institution: | (1) Department of Medical Microbiology, University of Linköping, S-581 Linköping, Sweden |
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Abstract: | The display of carbohydrate structures was measured in promyelocytic HL60 cells and in histiocytic U937 cells induced to differentiate to phagocytic cellsin vitro during three to seven days of cultivation in the presence of dimethylsulfoxide (DMSO). It was assessed by micro-or spectrofluorometric quantification of the binding of fluorescent lectins. Changes in the cell size and the association and uptake of IgG-or complementopsonized yeast cells (Saccharomyces cerevisiae) were used as signs of phagocyte differentiation.The binding of wheat germ agglutinin (WGA), concanavalin A (Con A),Ricinus communis agglutinin-I (RCA-I) andUlex europaeus agglutinin-I (UEA-I) varied due to the presence of DMSO during cultivation, and without DMSO also on the number of days in culture and the type of cell.Abbreviations DMSO
dimethylsulfoxide
- PMA
phorbol 12-myristate 13-acetate
- KRG
Krebs-Ringer phosphate buffer with glucose
- WGA
wheat germ agglutinin
- Con A
concanavalin A
- RCA-I
Ricinus communis agglutinin-I
- UEA-I
Ulex europaeus agglutinin-I |
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Keywords: | HL60 and U937 cells phagocytosis lectin binding microfluorometry |
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