Optimizing the shoot proliferation protocol of Penthorum chinense by axillary buds |
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Authors: | Li-Qiang Wang Jun Yang E. Deng Guang-Bi Wang Zheng-Song Peng |
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Affiliation: | (1) College of Life Sciences, China West Normal University, Nanchong, Sichuan, 637009, People’s Republic of China |
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Abstract: | Four parameters, three hormones and sucrose, at seven concentrations, were designed for shoot proliferation of Penthorum chinense by uniform design. The obtained data were used for building two quadratic polynomial equations by partial least square to determine optimum concentrations of four factors. Experiments for verification confirmed that no significant difference existed between the predicted and the validated values in shoot number and length based on all inoculated explants. |
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Keywords: | Axillary buds Medicinal herb Optimum culture medium Penthorum Shoot proliferation Uniform design |
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