Cloning vectors and antibiotic-resistance markers for Brevibacterium sp. R312 |
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| Authors: | C K Chion R Duran A Arnaud P Galzy |
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| Affiliation: | Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, ENSA, Montpellier, France. |
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| Abstract: | Replication of several cryptic plasmids from coryneform strains was investigated in Brevibacterium sp. R312. Only the Corynebacterium glutamicum pSR1 replicon was found to be suitable for establishing a host-vector system. Two pSR1 derivatives, pRPCG200 and pHYCG1, were used as cloning vectors. They carry a neomycin-resistance-encoding and a tetracycline-resistance-encoding gene, respectively. |
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