Expression of an apoplast-directed, T-phylloplanin-GFP fusion gene confers resistance against Peronospora tabacina disease in a susceptible tobacco |
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Authors: | Antoaneta B. M. Kroumova Dipak K. Sahoo Sumita Raha Michael Goodin Indu B. Maiti George J. Wagner |
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Affiliation: | 1. Kentucky Tobacco Research and Development Center, College of Agriculture, University of Kentucky, Lexington, KY, 40546, USA 2. Department of Radiation Oncology, Feinberg School of Medicine, Northwestern University, Ward- 13-002,303 East Chicago Ave, Chicago, IL, 60611, USA 3. Department of Plant Pathology, University of KY, 201F Plant Science Building, Lexington, KY, 40546, USA 4. Department of Plant and Soil Science, Kentucky Tobacco Research and Development Center, College of Agriculture, University of Kentucky, Lexington, KY, 40546, USA
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Abstract: | Key message Phylloplanins are plant-derived, antifungal glycoproteins produced by leaf trichomes. Expression of phylloplanin-GFP fusion gene to the apoplast of a blue mold susceptible tobacco resulted in increased resistance to this pathogen. Abstract Tobaccos and certain other plants secrete phylloplanin glycoproteins to aerial surfaces where they appear to provide first-point-of-contact resistance against fungi/fungi-like pathogens. These proteins can be collected by water washing of aerial plant surfaces, and as shown for tobacco and a sunflower phylloplanins, spraying concentrated washes onto, e.g., turf grass aerial surfaces can provide resistance against various fungi/fungi-like pathogens, in the laboratory. These results suggest that natural-product, phylloplanins may be useful as broad-selectivity fungicides. An obvious question now is can a tobacco phylloplanin gene be introduced into a disease-susceptible plant to confer endogenous resistance. Here we demonstrate that introduction of a tobacco phylloplanin gene—as a fusion with the GFP gene—targeted to the apoplasm can increase resistance to blue mold disease in a susceptible host tobacco. |
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