Immunolocalization of H+-ATPases in the plasma membrane of pollen grains and pollen tubes ofLilium longiflorum |
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Authors: | G. Obermeyer M. Lützelschwab H. -G. Heumann M. H. Weisenseel |
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Affiliation: | (1) Botanisches Institut I, Universität Karlsruhe, Karlsruhe;(2) Institut für Biologie III, Universität Freiburg, Freiburg;(3) Present address: Institut für Pflanzenphysiologie, Universität Salzburg, Hellbrunner Strasse 34, A-5020 Salzburg, Austria |
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Abstract: | Summary A heterogeneous distribution of H+-ATPase was visualized in germinated pollen ofLilium longiflorum using monoclonal antibodies raised against plasma membrane H+-ATPase. Immunolocalization studies of protoplasts and subprotoplasts derived from pollen tubes and sectioned pollen grains and pollen tubes show that H+-ATPases are abundant in the plasma membrane of pollen grains but are absent or sparsely distributed in the plasma membrane of pollen tubes. This polar distribution of H+-ATPases is probably the basis of the endogenous current pattern measured in growing lily pollen and involved in pollen tube tip growth.Abbreviations BSA bovine serum albumine - Hepes N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid - Mes 2-(N-morpholino)-ethane sulphonic acid - PBS phosphate buffered saline - Pipes piperazine-N,N-bis(2-ethanesulfonic acid) - Tris 2-amino-2-hydroxymethyl-1,3-propandiol |
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Keywords: | Immunolocalization H+-ATPase Tip growth Lilium longiflorum |
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