| 蓝猪儿组织培养和再生 |
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| 引用本文： | 李玲,黄群声,张铭光,宾金华.蓝猪儿组织培养和再生[J].亚热带植物科学,2001,30(4):27-31. |
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| 作者姓名： | 李玲 黄群声 张铭光 宾金华 |
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| 作者单位： | 华南师范大学 生物系, 广东 广州 510631 |
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| 基金项目： | 广东省自然科学基金项目(003062) |
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| 摘 要： | 光照有利于蓝猪儿(Torenia fournieri)种子的萌发。上胚轴转接在生芽培养基(MS+0.1 mg/L 4PU+2 mg/L NAA)上,25℃,光暗16/8h培养18d,芽发生,25d不定芽数目达7~8个。叶或茎形成愈伤组织的适宜培养基为MS+0.05 mg/L 2,4-D+0.2 mg/L NAA,愈伤组织再分化形成芽的能力较弱。1/2 MS培养基无论是否添加萘乙酸,蓝猪儿试管苗都能发生不定根,萘乙酸使不定根的数目增多,分布密集。
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| 关 键 词： | 蓝猪儿 组织培养 |
| 文章编号： | 1009-7791(2001)04-0027-05 |
| 收稿时间： | 2001-07-04 |
| 修稿时间： | 2001年7月4日 |
Tissue culture and regeneration of Toreniafournieri |
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| LI Ling,HUANG Qun-sheng,ZHANG Ming-guang,BIN Jin-hua.Tissue culture and regeneration of Toreniafournieri[J].Subtropical Plant Science,2001,30(4):27-31. |
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| Authors: | LI Ling HUANG Qun-sheng ZHANG Ming-guang BIN Jin-hua |
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| Institution: | Department of Biology, South China Normal University, Guangzhou 510631, Guangdong China |
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| Abstract: | Illumination could promote the germination of Torenia fournieri seeds. Adventitious buds were produced from epicotyl on MS medium containing 4PU at 0.1mg/L, NAA at 2mg/L with a 16h photoperiod for 18 days at 25℃. 7~8 adventitious buds were obtained after 25 days of culture. A combination of MS added with 2,4-D at 0.05mg/L and NAA at 0.2mg/L gave the best result for leaf and stem to induce calli, but the callus differentiation to buds was difficult. The tube plantlet cultured on 1/2 MS medium could produce roots and NAA was optimum for root number. |
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| Keywords: | Torenia fournieri tissue culture |
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