Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses |
| |
Authors: | Pulla Rama Krishna Kim Yu-Jin Kim Myung Kyum Senthil Kalai Selvi In Jun-Gyo Yang Deok-Chun |
| |
Affiliation: | Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Seocheon-dong, Kiheung-gu Yongin, Kyunggi-do, South Korea. |
| |
Abstract: | Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|