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Signature fatty acids in the polar lipids of acid-producing Thiobacillus spp.: Methoxy, cyclopropyl, alpha-hydroxy-cyclopropyl and branched and normal monoenoic fatty acids
Authors:Brent D. Kerger  Peter D. Nichols  Christopher P. Antworth  Wolfgang Sand  Eberhard Bock  John C. Cox  Thomas A. Langworthy  David C. White
Affiliation:Department of Biological Science, Florida State University, Tallahassee, FL 32306-3043, U.S.A.;Institute for General Botany and Botanical Garden, University of Hamburg, Hamburg, F.R.G.;Martek Corporation, Columbia, MD, U.S.A.;Department of Microbiology, University of South Dakota, Vermillion, SD, U.S.A.
Abstract:Abstract The polar lipids of 5 species of Thiobacillus were extracted and purified. An analysis of the fatty acid composition of the polar lipids documented the presence of methoxy, cyclopropyl, monounsaturated and hydroxycyclopropyl fatty acids of sufficiently unusual structure to serve as 'signatures' for the presence of these organisms in environmental samples. The structures of the unusual fatty acids of the polar lipids were confirmed by mass spectrometry (MS) after isolation by capillary gas chromatography (GC).
Keywords:Polar lipids    fatty acids    Thiobacillus ssp.    acid-producing bacteria    hydroxycyclopropyl fatty acids    methoxy fatty acids
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