Improved Visualization of Cell Nuclei in Unstained Cultured Cells |
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Authors: | Ricky J. Ballou Ahmad R. Safa Michael T. Tseng |
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Affiliation: | a Department of Anatomy and The Regional Cancer Center, University of Louisville Health Sciences Center, Louisville, Kentucky |
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Abstract: | Nuclear morphology is useful in tissue culture studies in determining the presence and grade of transformed cells as well as in determining the heterogeneity of the cell population (Grogan el al. 1981, Hustin 1976, Siracky et al. 1978, Siracky 1979). The ratio of long and short nuclear axes provides a useful numerical expression of nuclear shape (Hustin 1976). Clear visualization of nuclei is critical for making the necessary measurements. |
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