Rad9/53BP1 protects stalled replication forks from degradation in Mec1/ATR‐defective cells |
| |
Authors: | Matteo Villa Diego Bonetti Massimo Carraro Maria Pia Longhese |
| |
Institution: | Dipartimento di Biotecnologie e Bioscienze, Università di Milano‐Bicocca, Milan, Italy |
| |
Abstract: | Nucleolytic processing by nucleases can be a relevant mechanism to allow repair/restart of stalled replication forks. However, nuclease action needs to be controlled to prevent overprocessing of damaged replication forks that can be detrimental to genome stability. The checkpoint protein Rad9/53BP1 is known to limit nucleolytic degradation (resection) of DNA double‐strand breaks (DSBs) in both yeast and mammals. Here, we show that loss of the inhibition that Rad9 exerts on resection exacerbates the sensitivity to replication stress of Mec1/ATR‐defective yeast cells by exposing stalled replication forks to Dna2‐dependent degradation. This Rad9 protective function is independent of checkpoint activation and relies mainly on Rad9‐Dpb11 interaction. We propose that Rad9/53BP1 supports cell viability by protecting stalled replication forks from extensive resection when the intra‐S checkpoint is not fully functional. |
| |
Keywords: | Mec1 Rad9 replication forks resection Sgs1 |
|