Protein synthesis in vitro in a system from plant mitochondria (Short Communication) |
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| Authors: | Biswendu B Goswami Symamalima Chakrabarti Dipak K Dube and S C Roy |
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| Institution: | Department of Biochemistry, University College of Science, 35 Ballygunge Circular Road, Calcutta-19, India |
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| Abstract: | A mitochondrial system from 48h-germinating seeds of Vigna sinensis (Linn.) Savi is capable of incorporating l-U-(14)C]valine into proteins and is practically insensitve to cycloheximide, but highly sensitive to chloramphenicol and fusidic acid, a potent inhibitor of peptide-chain elongation factor. A system consisting of mitochondrial S-100 fraction and ribosomes from the same source and other cofactors is capable of polyphenylalanine synthesis and behaves similarly with respect to these inhibitors. |
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