Occurrence of a Large Ca2+-Independent Release of Glutamate During Anoxia in Isolated Nerve Terminals (Synaptosomes) |
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Authors: | Jose Sanchez-Prieto Pilar González |
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Institution: | Instituto de Bioquímica, Centro Mixto C.S.I.C.-U.C.M., Facultad de Farmacía, Universidad Complutense, Madrid, Spain. |
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Abstract: | Isolated rat cerebral cortical synaptosomes made anoxic by addition of cyanide developed an inhibition of the Ca2+-dependent release of glutamate 2 min after the addition of the metabolic inhibitor when the intrasynaptosomal ATP/ADP ratio decreased below 1.7. In contrast, cyanide induced a continuous efflux of glutamate through a Ca2+-independent pathway that accounted for the release of 25% of total intrasynaptosomal glutamate in 5 min. The results suggest that a Ca2+-independent release of glutamate could be implicated in the neurotoxic action of this amino acid during anoxia. |
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Keywords: | Glutamate Anoxia Synaptosomes Exocytosis ATP Ca |
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