冬虫夏草菌丝状菌源增殖培养的研究

冬虫夏草菌是珍稀濒危名贵中药材冬虫夏草的无性型菌种,是侵染蝠蛾幼虫的唯一菌种,其侵染后形成名贵中药材冬虫夏草。冬虫夏草菌在不同营养及条件下生长形态不同,主要有丝状体和菌球体两种生长形态。冬虫夏草菌丝状菌体能够侵染蝠蛾幼虫,有可能作为一种新的接种体被广泛应用;但丝状菌体对营养要求苛刻、生长缓慢、菌丝体得率低的特点,阻碍了冬虫夏草菌丝状菌体作为侵染蝠蛾幼虫的接种体的开发及应用,从而阻碍了冬虫夏草人工培殖产业化的进程。为了提高冬虫夏草菌丝状菌体的生物表达量,本文对营养及培养条件进行了优化研究,得出了最佳条件为:葡萄糖质量浓度40 g/L、酵母粉质量浓度65 g/L、培养温度17℃、MgSO_4质量浓度3 g/L、KH_2PO_4质量浓度1.5 g/L、培养时间12 d,按最优培养条件,冬虫夏草菌丝状菌体干重得率15.67 g/L,为下一步新的接种体的制备提供菌源保障。

The research on the proliferative culture of Ophiocordyceps sinensis filamentous mycelium

The herb of Ophiocordyceps sinensis is that genus Hepialus caterpillars were infected by parasitic fungus O. sinensis to form a combination of insect and fungi, which it is precious and specific natural resources in China. The asexual stage of the herb of O. sinensis was O. sinensis which has has two kinds of morphology—the filamentous and pellet mycelium under different nutritional conditions. Moreover, only the filamentous mycelium can infect the Hepialus caterpillars, so it may be applied widely as a new inoculant. However, the defect of slow growth and low yield from the filamentous mycelium of O. sinensis has hindered its application in the inoculant of the Hepialus caterpillars, and the modern industrial cultivation. In order to improve the yield of the O. sinensis mycelium, the nutritional conditions were optimized by orthogonal experiment design. The results showed that the yield of filamentous mycelium reached 15. 67 g/L under the conditions of glucose 40 g/L, yeast powder 65 g/L, temperature 17℃, KH2 PO4 1. 5 g/L, MgSO4 3 g/L and incubation time 12 days. The optimized yield of filamentous mycelium in this study will provide a large of raw materials for the prepared Hepialus moths larva inoculant.