高温对西花蓟马卵巢发育及卵黄蛋白含量的影响

【目的】为探明短时高温热激后西花蓟马 Frankliniella occidentalis (Pergande)生殖力和卵巢发育的相互关系。【方法】选择1龄、2龄若虫,预蛹,蛹和1日龄成虫，采用不同高温（41和45℃）短时（2 h）处理，观察卵巢发育及卵黄蛋白相对含量的变化。【结果】高温热激主要抑制了西花蓟马生殖器官和胚胎的正常发育，导致卵巢管数量减少，卵巢管和胚胎发育畸形；电泳结合不同的染色方法证明西花蓟马的卵黄蛋白为一种磷酸化、糖基化的脂蛋白，由3个亚基组成，分子量分别为116，70和47 kDa。除热激蛹期外，热激其他龄期的若虫对其当代成虫卵黄蛋白合成都起到抑制作用，且45℃热激1龄或2龄若虫后，其对卵黄蛋白相对含量的影响持续到了F₁代。【结论】温度对雌虫生殖力的影响是由于其对生殖器官发育和卵黄蛋白合成的影响引起的。本研究为西花蓟马的卵黄蛋白和卵黄发生的进一步研究奠定了基础。

Effects of high temperature on ovary development and yolk protein content of Frankliniella occidentalis (Thysanoptera: Thripidae)

【Aim】 This study aims to clarify the relationship between fecundity and ovary development of Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). 【Methods】 The 1st instar nymphs, 2nd instar nymphs, prepupae, pupae, and the 1-day-old adults were subjected to 41℃ and 45℃ heat shock, respectively, for 2 h and the changes in their ovary development and relative contents of yolk protein were observed. 【Results】 The normal development of reproductive organs and embryos of F. occidentalis were inhibited by heat exposure, which resulted in the reduction in the number of ovarian tubes and deformity of ovarian tubes and embryo. The yolk protein of F. occidentalis was identified as a phosphorylated and glycosylated lipoprotein, consisting of three subunits with the molecular weight of 116, 70 and 47 kDa, respectively, through electrophoresis and different dyeing methods. The yolk protein synthesis of F₀ generation adults was inhibited when nymphs and prepupae were exposed to high temperature, and the effect of high temperature on the relative content of yolk protein continued to F₁ generation whenthe 1st or 2nd instar nymphs were exposed to high temperature (45℃). 【Conclusion】 The effect of temperature on female fecundity of F. occidentalis is due to its influence on the development of reproductive organs and the synthesis of yolk protein. This study lays a foundation for the further research on yolk protein and vitellogenesis of F. occidentalis.