基于16S rDNA基因序列的泽兰实蝇幼虫肠道细菌多样性分析

【目的】探究泽兰实蝇 Procecidochares utilis 幼虫肠道细菌的多样性。【方法】利用Illumina HiSeq技术对泽兰实蝇幼虫肠道细菌的16S rDNA-V6变异区序列进行测序，应用USEARCH和QIIME等软件整理和统计样品序列数目和操作分类单元（operational taxonomic unit, OTU）数量，分析物种的丰度和Alpha多样性。【结果】共获得1 593 506 对reads，拼接为1 579 372条tags，经过滤后得到的1 572 860条tags聚类为1 341个OTU。总共注释到13个门，4个纲，6个目，7个科，10个属和4个种。其中变形菌门(Proteobacteria)的细菌为优势菌，占99%；在属分类阶元上，沃尔巴克氏体属 Wolbachia 占45%，是优势属。【结论】泽兰实蝇幼虫肠道细菌多样性丰富。相关的种类和丰度信息为后期研究揭示肠道细菌介导泽兰实蝇寄主植物专化性奠定了基础。

Analysis of the bacterial diversity in the intestine of larval Procecidochares utilis (Diptera: Trypetidae) based on 16S rDNA gene sequence

【Aim】 To clarify the bacterial diversity in the intestine of larval Procecidochares utilis. 【Methods】 The V6 regions of the 16S rDNA genes of intestinal bacteria in P. utilis larvae was sequenced by Illumina HiSeq techniques. The numbers of sequences and operational taxonomic units (OTUs), species abundance and alpha diversity in samples were analyzed using USEARCH and QIIME softwares. 【Results】 A total of 1 593 506 reads were obtained, which were combined to 1 579 372 tags. After filtration, 1 572 860 tags were clustered into 1 341 OTUs. They were annotated into 13 phyla, 4 classes, 6 orders, 7 families, 10 genera, and 4 species. The Proteobacteria was the most dominant, accounting for 99%. At the genus level, Wolbachia was the dominant bacteria(45%). 【Conclusion】 The results show that the bacteria in the intestine of larval P. utilis are diverse. The information of species richness will lay a foundation for further research on the association of the intestinal bacteria with host plant to reveal feeding specialization in P. utilis.