宽翅曲背蝗受精囊形态与超微结构观察

[目的]明确宽翅曲背蝗Pararcyptera microptera meridionalis雌虫受精囊的形态、组织结构与超微结构,为更好地认识昆虫受精囊的功能提供依据.[方法]本研究以宽翅曲背蝗已交配雌成虫为实验材料,利用光学显微镜和透射电子显微镜观察其受精囊的形态、组织结构和超微结构.[结果]宽翅曲背蝗受精囊由一个端囊和一条长的受精囊管组成,端囊用于储存精子.端囊和受精囊管有相似的组织学结构,由外到内依次为肌肉层、基膜、上皮层及表皮内膜.上皮层含上皮细胞、腺细胞和导管细胞3种细胞类型.腺细胞具有一个被有微绒毛的细胞外腔.腺细胞的分泌物经细胞外腔通过分泌导管进入到受精囊腔.分泌导管由导管细胞形成.[结论]在宽翅曲背蝗受精囊的端囊和受精囊管上,内膜和腺细胞的细胞外腔结构均存在差异,由此推测,端囊和受精囊管的功能存在一定差异.上皮细胞的超微结构特点显示上皮细胞具有支持、分泌和吸收的功能.

Morphology and ultrastructure of the spermatheca of Pararcyptera microptera meridionalis (Orthoptera: Arcypterinae)

[Aim] To ascertain the morphology,organization structure and ultrastructure of the spermatheca in females of Pararcyptera microptera meridionalis (Orthoptera:Arcypterinae) for providing a better understanding of the functions of insect spermatheca.[Methods] The morphology,organization structure and ultrastructure of spermatheca of mated female adults of P.m.meridionalis were examined by means of the light and transmission electron microscopy.[Results] The spermatheca is composed of a seminal receptacle,mainly serving for storage of spermatozoa,and a long spermathecal duct.Histologically,both parts have an outer muscle coat followed by a basal lamina,epithelium,and cuticular intima.The epithelium is made up of three cell types,i.e.,epithelial cells,gland cells and duct cells.Each gland cell has a reservoir lined with microvilli.The secretions of gland cell are released into the reservoir through which it reaches the spermathecal lumen via the secretory duct formed by the duct cell.[Conclusion] Regional differences in the ultrastructure of the cuticular intima and the reservoir in gland cell imply that the seminal receptacle and the spermathecal duct carry out different functions.The ultrastructural features revealed that epithelial cells are involved in the function of support,secretion and absorption.