A novel method for immobilization of invertase from the thermophilic fungus, Thermomyces lanuginosus |
| |
Authors: | SY Basha P Palanivelu |
| |
Institution: | (1) Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India;(2) Present address: Department of Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA;(3) Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India |
| |
Abstract: | An invertase from the thermophilic fungus, Thermomyces
lanuginosus was immobilized on phenyl-Sepharose and its properties were studied. Between the soluble and immobilized forms of the invertase, there were not much difference in their optimum pH, K
M and V
max for sucrose. In contrast, the K
M and V
max for raffinose changed significantly. The optimum temperature for the immobilized invertase was lower by 10 C. The immobilized invertase showed remarkable stability at 50 C and was less sensitive to inhibition by metal ions. There was no leaching of the enzyme for at least a month when stored in the refrigerator. The method is novel and specific for the thermophilic invertase as a mesophilic invertase (from yeast) did not bind to phenyl-Sepharose. |
| |
Keywords: | Invertases immobilization phenyl-Sepharose thermophilic fungus yeast |
本文献已被 SpringerLink 等数据库收录! |
|