Micropropagation of Raisin Tree (Hovenia dulcis Thunb.) Through Axillary Bud Culture |
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Authors: | Sergio Echeverrigaray Altemir Jose Mossi Fernanda Munari |
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Institution: | 1. Divisao de Biotecnologia Vegetal, Instituto de Biotecnologia, Universidade de Caxias do Sul, CxP 1352, CEP 97001-970, Caxias do Sul, RS, Brazil
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Abstract: | Clonal propagation in vitro of raisin tree (Hovenia dulcis Thunb.) was achieved using axillary buds from mature trees and young plants. Explants cultured on Murashige-Skoog’s medium with 1/3 of the original salt concentration, supplemented with 0.5 mg l-1 BAP and 0.5 mg l-1 IAA, showed proliferation of new shoots in 4-5 weeks. Adventitious shoot proliferation was also stimulated in subsequent subcultures in the presence of BAP. The shoots rooted when transferred to 1/3 Murashlge and Skoog’s medium with 0.1 mg l-1 of IBA. Plantlets thus formed were successfully transplanted to the field after a short acclimatization period. |
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