Global analysis of non-coding small RNAs in Arabidopsis in response to jasmonate treatment by deep sequencing technology |
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Authors: | Zhang Bosen Xie Daoxin Jin Zhiping |
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Institution: | School of Life Sciences, Tsinghua University, Beijing 100084, China. |
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Abstract: | In plants, non-coding small RNAs play a vital role in plant development and stress responses. To explore the possible role of non-coding small RNAs in the regulation of the jasmonate (JA) pathway, we compared the non-coding small RNAs between the JA-deficient aos mutant and the JA-treated wild type Arabidopsis via high-throughput sequencing. Thirty new miRNAs and 27 new miRNA candidates were identified through bioinformatics approach. Forty-nine known miRNAs (belonging to 24 families), 15 new miRNAs and new miRNA candidates (belonging to 11 families) and 3 tasiRNA families were induced by JA, whereas 1 new miRNA, 1 tasiRNA family and 22 known miRNAs (belonging to 9 families) were repressed by JA. |
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Keywords: | Non-coding small RNAs jasmonate Arabidopsis Solexa deep sequencing |
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