Influence of growth rate on pigment and lipid composition of the microalgaIsochrysis aff.galbana clone T.iso |
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Authors: | L. Saoudi-Helis J. -P. Dubacq Y. Marty J. -F. Samain C. Gudin |
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Affiliation: | (1) IFREMER, Centre de Brest, Laboratoire de recherches aquacoles, BP 70, 29280 Plouzané, France;(2) Ecole Normale Supérieur, Laboratoire de Biomembranes et Surfaces Cellulaires, CNRS URA 311/GDR 1002, 46 rue d'Ulm, Paris, France;(3) CNRS/URA 322, Faculté des Sciences et Techniques, 6 Avenue Le Gorgeu, Brest, France;(4) CEA Cadarache, Laboratoire Héliosynthèse, BP 1, 13108 Saint Paul-Lez-Durance Cedex, France |
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Abstract: | A continuous culture ofIsochrysis aff.galbana clone T.iso, used to feedPecten maximus larvae at IFREMER (Brest, France), was carried out in a chemostat at its optimum temperature for growth (26 °C). Changes in pigments, lipid class (neutral, glyco- and phospholipids) and degree of fatty acid unsaturation were studied at three different growth rates (0.33, 0.5, 1 d–1). As predicted by chemostat theory, a slow growth rate produced higher cell numbers and higher biomass per unit volume. These cells were low in chlorophylla and carotenoids, but rich in neutral lipids. In contrast, cultures with a fast growth rate yielded lower cell concentrations, buth higher chlorophylla, carotenoid and membrane lipid contents per cell. Changes in polyunsaturated fatty acid distribution were related to differences in algal growth rates. Neutral lipids contained mainly saturated and monounsaturated fatty acids (C18:19) at low growth rates whereas they were enriched in polyunsaturated fatty acids, especially C22:63, at high growth rates. Therefore, it is suggested that the growth rate in continuous cultures be controlled so as to adjust the relative proportions of polyunsaturated fatty acids in lipid classes of the diet meant for larval nutrition.Author for correspondence |
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Keywords: | microalgae Isochrysis aff.galbana clone T.iso lipids fatty acids growth rate |
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