重症肌无力小鼠模型的建立

目的建立重症肌无力小鼠模型。方法电鳗乙酰胆碱受体(TnAChR)和完全弗氏佐剂(CFA)混合物免疫C57BL/6J小鼠,经二次加强免疫后,检测肌力、腓肠肌肌电图、膈肌终板电镜、血清抗体水平等指标。结果与对照小鼠相比,发病小鼠表现出肌力减弱的症状,肌电图显示小鼠腓肠肌复合动作电位振幅幅度显著下降,电镜证实神经肌接头处突触后膜变平、皱褶减少、空泡样变性,发病小鼠血清抗体水平明显升高。结论成功建立了重症肌无力小鼠模型,有助于探讨其发病机理及探索治疗自身免疫病新的途径和方法。

Establishment of a Mouse Model of Experimental Autoimmune Myasthenia Gravis

Objective To develop a mouse model of myasthenia gravis.Methods C57BL/6J mice were immunized with the mixture of acetylcholine receptor extracted from Torpedo Californica and complete Freund's adjuvant.After twice strengthened immunization,several indexes including myodynamia,electromyogram,diaphragma end-plate electron microscopy and serum anti-nAChR level were tested.Results Compared with the control,the myasthenic mice displayed myodynamia-attenuating,and the electromyogram showed an apparently declined amplitude of gastrocnemius combined action potential.The electron microscopic examination demonstrated flattened postsynaptic membrane,its folds diminished and showed vacuolar degeneration.The serum antibody level in the myasthenic mice increased significantly.Conclusion We have successfully established a mouse model of myasthenia gravis,which may be helpful in investigation of mechanisms of MG and pursue new ways to treat autoimmune diseases.